Cattle GnRH (Gonadotropin Releasing Hormone) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0603-48, ELK0603-96, ELK0603-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: GNRH1; GRH; LNRH; LHRH; LH-RH I; Luliberin; Luteinizing-Hormone Releasing Hormone; Progonadoliberin-1; Gonadoliberin I; Gonadorelin; GnRH-associated peptide 1

Assay Type: Competitive Inhibition

Sensitivity: 23.4 pg/mL

Standard: 4000 pg/mL

Detection Range: 62.50-4000 pg/mL

Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2.5h

Research Areas: Endocrinology; Reproductive science; Neuroscience; Genetic science; Hormone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle GnRH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle GnRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle GnRH in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only