The nematodes enter the pest and release symbiotic bacteria into the pest's body cavity. These bacteria convert the host tissue into a food source, on which nematodes feed, develop, and reproduce inside the host. This kills the pest within a few hours to days after infection.

• Remove the sachets from the box and keep them at room temperature for 30 minutes
• Empty the contents of the sachets into a bucket containing at least 2 litres of water per sachet (water temperature: 15-20°C/59-68°F)
• Stir well and leave the contents to soak for five minutes
• Stir again and pour the contents of the bucket into the half-filled spray tank (except when tank mixing with a compatible product)
• Maintain agitation of mixture in the tank (e.g. with a recirculation pump)
• Fill the spray tank with the required amount of water
• If tank mixing with a compatible product, add Entonem at the end to a fully filled tank
• Apply directly after preparation of spray solution

• Nematodes can be applied using a watering can, an airblast sprayer, through a sprinkler system, with a backpack sprayer, or a vehicle-mounted spray unit
• To avoid blockage, all filters should be removed, especially if finer than 0.3 mm (50 mesh)
• Use a maximum pressure of 20 bar/290 psi (on nozzle)
• The spray nozzle opening should be at least 0.5 mm (500 microns – 35 mesh) - preferably high flow rate cone nozzle types
• Continuous mixing should take place to prevent nematodes sinking to the bottom of the spray tank
• Avoid sprayers equipped with centrifugal or piston pumps
• Water tank temperature must not exceed 25°C/77°F and pH must be between 4 and 8
• Distribute the spraying solution evenly over the soil/medium surface