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Cyanophycean MediumCyanophycean Medium Suitable for blue green algae. Directions For 1 L Total To approximately 975 mL of dH2O add each of the components in the order specified while stirring continuously. Bring the total volume to 1 L with dH2O. For 1. 5% agar medium add 15 g of agar into the flask; do not mix. Cover and autoclave medium. Store at refrigerator temperature. # Component Amount Stock Solution Concentration Final Concentration 1 P IV Metal Solution 6 mL L
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Cyanophycean Medium

Suitable for blue-green algae.

Directions

For 1 L Total
  1. To approximately 975 mL of dH2O add each of the components in the order specified while stirring continuously.
  2. Bring the total volume to 1 L with dH2O.
    • For 1.5% agar medium add 15 g of agar into the flask; do not mix.
  3. Cover and autoclave medium.
  4. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 P-IV Metal Solution 6 mL/L
2 KNO3
(Baker 3190)
5 g/L 50 mM
3 K2HPO4
(Sigma P 3786)
0.05 g/L 0.29 mM
4 MgSO47H2O
(Sigma 230391)
0.05 g/L 0.2 mM

Cyanophycean Medium

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