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LDM MediumLDM Medium Modification of Lewin's marine diatom medium. Directions For 1 L Total To approximately 950 mL of seawater (30 ppt), add each of the components in the order specified (except vitamins) while stirring continuously. Bring total volume to 1 L with non pasteurized seawater. For 1. 5% (w v) agar medium, add 15 g of agar into the flask; do not mix. Cover and autoclave medium. When cooled add sterile vitamins. For agar medium add vitamins, mix,

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LDM Medium

Modification of Lewin's marine diatom medium.

Directions

For 1 L Total

To approximately 950 mL of seawater (30 ppt), add each of the components in the order specified (except vitamins) while stirring continuously.
Bring total volume to 1 L with non-pasteurized seawater.
- For 1.5% (w/v) agar medium, add 15 g of agar into the flask; do not mix.
Cover and autoclave medium.
When cooled add sterile vitamins.
- For agar medium add vitamins, mix, and dispense before agar solidifies.
Store at refrigerator temperature.

#	Component	Amount	Stock Solution Concentration	Final Concentration
1	Seawater (30 ppt)	950 mL
2	NaNO₃ (Fisher BP360-500)	1 mL/L	10 g/400mL dH2O	0.29 mM
3	CaCl₂•2H₂O (Sigma C-3881)	1 mL/L	1 g/400mL dH2O	0.017 mM
4	MgSO₄•7H₂O (Sigma 230391)	1 mL/L	3 g/400mL dH2O	0.03 mM
5	K₂HPO₄ (Sigma P 3786)	1 mL/L	3 g/400mL dH2O	0.043 mM
6	KH₂PO₄ (Sigma P 0662)	1 mL/L	7 g/400mL dH2O	0.129 mM
7	NaCl (Fisher S271-500)	1 mL/L	1 g/400mL dH2O	0.043 mM
8	P-IV Metal Solution	6 mL/L
9	Tryptone (Sigma T 9410)	1 g/L
10	Vitamin B₁₂	1 mL/L
11	Biotin Vitamin Solution	1 mL/L

LDM Medium

Name: LDM Medium
Brand: wellness-welten.net
SKU: 19842767897
Price: 60.00 USD
Availability: InStock
Rating: 4.198 (2399 reviews)