CD56 Monoclonal Antibody

Product: Mouse IgG. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Catalogue Numbers: MB66153-50, MB66153-100

Sizes: 50µl, 100µl

Swiss-Prot: P13591

Host: Mouse

Reactivity: Human

Applications: WB, IHC

Background: NCAM (neural cell adhesion molecule, CD56) is an adhesion glycoprotein with five extracellular immunoglobulin-like domains followed by two fibronectin type III repeats. Structural diversity is introduced by alternative splicing resulting in different cytoplasmic domains . NCAM mediates neuronal attachment, neurite extension, and cell-cell interactions through homo and heterophilic interactions. PSA (polysialic acid) post-translationally modifies NCAM and increases the metastatic potential of small cell lung carcinoma, Wilms+ tumor, neuroblastoma, and rhabdomyosarcoma . CD56 is commonly used along with CD3 and CD16 to identify human NK cells (Mouse NK cells do not express CD56) . Human natural killer cells are CD3-CD56+. The large subset with high CD16 expression are mature cytotoxic natural killer cells, while those with low CD16 expression are immature precursors and cytokine producers .

Product: Mouse IgG. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of CD56 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CD56 expression in Hela (A) whole cell lysates., Immunohistochemical analysis of CD56 staining in human small cell lung carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 215 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: NCAM; Neural cell adhesion molecule 1; N-CAM-1; NCAM-1; CD56

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human CD56. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified