BL21(λDE3) pLPP Competent Cells for Unphosphorylated Protein Production

Undesired protein phosphorylation by host kinases or autophosphorylation often complicates protein expression in standard bacterial strains. The BL21(λDE3) pLPP E. coli strain is specifically engineered for high-efficiency, homogeneous expression of non-phosphorylated proteins—including challenging serine/threonine kinases and tyrosine kinases.

How It Works

Amid Biosciences' BL21(λDE3) pLPP chemically competent cells contain an IPTG-inducible bacteriophage lambda protein phosphatase (lambda PP) expression plasmid. Lambda PP is a highly specific enzyme that efficiently removes phosphorylation from Ser, Thr, and Tyr residues in target proteins during co-expression.

Key Features & Advantages

• Broad Compatibility: The lambda PP plasmid carries a streptomycin/spectinomycin resistance gene, allowing seamless co-expression with common T7 promoter-driven vectors (e.g., pET, pTrc).
• High Efficiency: Yields homogeneously dephosphorylated targets, simplifying downstream characterization and structural analysis.
• Ready-to-Use Format: Supplied as a convenient package of 10 individual 100 μl/tube aliquots to eliminate freeze-thaw degradation.

Technical Specifications

• E. coli Genotype: fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS pLPP (StrR) [λDE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5]
• Transformation Efficiency: >1 x 10^4 cfu/µg using pBR322
• Format: Chemically Competent Cells (10 x 0.1 mL)
• Storage Conditions: Store strictly at -80°C (Do Not Freeze)

This product is intended for laboratory research use only.